Journal: Cancer Immunology, Immunotherapy : CII

Article Title: Characterization of the first fully human anti-TEM1 scFv in models of solid tumor imaging and immunotoxin-based therapy

doi: 10.1007/s00262-016-1937-z

Figure Lengend Snippet: ScFv78-mediated NP internalization. a Illustration of the components used to prepare scFv78-NP-dye and BSA-NP-dye. b Streptavidin-conjugated iron-oxide nanoparticles co-labeled with BSA and BSA-Alexa649 (BSA-NP) or scFv78 and BSA-Alexa649 (scFv78-NP). c FACS analysis of the fluorescent intensity of the non-labeled nanoparticles (red), BSA-NP (blue) and scFv78-NP (green). d scFv78-NP or BSA-NP was incubated with MS1 and MS1-TEM1 (TEM1+) cells, respectively, in complete medium, and then internalization was analyzed by confocal imaging

Article Snippet: Streptavidin-coated iron-oxide nanoparticles (NPs) (MiltenyiBiotec, CA) with a diameter of 50 nm were used for the specific internalization assay. scFv78 was site-specifically biotinylated in yeast to obtain scFv78-bio as described previously.

Techniques: Labeling, Incubation, Imaging

Journal: Proceedings - Society of Photo-Optical Instrumentation Engineers

Article Title: Iron Oxide Hyperthermia And Radiation Cancer Treatment

doi: 10.1117/12.810035

Figure Lengend Snippet: This bar graph represents the average number of days for the tumor volume to triple following treatment. Error bars represent standard error calculations. The nanoparticles plus radiation group averaged approximately 23 days tumor regrowth delay, whereas the iron oxide nanoparticles hypothermia group averaged approximately 8 days (41.5–43 °C /10 min, < 5 CEM) and the 15 Gy radiation alone group average approximate 16 days

Article Snippet: The nanoparticles plus radiation group averaged approximately 23 days tumor regrowth delay, whereas the iron oxide nanoparticles hypothermia group averaged approximately 8 days (41.5–43 °C /10 min, < 5 CEM) and the 15 Gy radiation alone group average approximate 16 days Thermometry shows that application of the IONP hyperthermia in our setting, does not increase mouse core temperature to unsafe levels.

Techniques: